eDNA Metabarcoding
James Hutton Institute Scientific Services can provide services from eDNA extraction through to provision of DNA sequences for bacterial and fungal diversity.
You can request the whole process, or just parts of the process.
The eDNA Metabarcoding we can provide:
eDNA extraction
Provide us with environmental sample (e.g. water, soil, sediment, etc.) and we can extract eDNA. We can provide information on quality, quantity and integrity. Long-term archiving of eDNA samples will come at an additional cost.
PCR and Library Prep
We can PCR amplify specific regions to characterise bacterial and fungal diversity. We can then make libraries ready for sequencing.
Sequencing
This will be undertaken at our Genomics Facility. We provide library QC and downstream processing up to the point of data generation. We sequence using NextSeq, providing 300 bp paired end.
Dr Eulyn Pagaling, Senior Environmental Microbiologist
eDNA metabarcoding is gaining popularity in biodiversity monitoring and assessment due to its ease of use, speed and cost-effectiveness.
Data Processing
We can carry out sequence processing including demultiplexing, trimming, merging, QC, denoising, chimera removal and clustering. We can provide OTU tables of identified taxa.
Open Data
We can archive scripts and results in a public repository to provide transparency in our processes.
If any of these services are of interest please contact us.
